Library prep
A single assay for comprehensive discovery of the 5-base genome (A, T, G, C, and 5mC), providing dual insights into the whole genome and methylome.
Sequencing
Perform powerful high-throughput sequencing studies. Match data output and time to results to project needs.
Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.
Data analysis
Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing (NGS) data.
A cloud-based multiomic analysis and interpretation software, enabling sample to insight workflow with visualizations, scalability, and secure data management.
Detect five DNA bases in a single assay while delivering concurrent genetic and epigenetic insights.
Sensitivity and precision plot for germline single nucleotide variant (SNV) calling shows Illumina 5-Base DNA Prep is very close to standard whole-genome sequencing (WGS) with Illumina DNA PCR-Free Prep, and higher than EM-Seq v2 and bisulfite sequencing.
Illumina 5-Base DNA Prep uses a one-step enzymatic process to convert only 5mC to T, yielding greater nucleotide diversity than traditional bisulfite treatment or enzymatic methylation sequencing (EM-Seq), in which unmethylated C are converted to T.
Illumina 5-Base DNA Prep offers highly accurate methylation measurement at CpG islands. Weighted methylation levels of individual CpG islands using Illumina 5-Base DNA Prep are highly concordant with EM-Seq v2 (left) and with bisulfite sequencing (right).
Illumina 5-Base DNA Prep shows ~20% more CpGs with at least 10× coverage compared to EM-Seq v2 and 68% more CpGs with at least 10× coverage compared to bisulfite sequencing for 450M clusters (900M paired-end reads).
Illumina 5-Base DNA Prep shows reduced GC bias with normalized coverage that is consistently higher at low-GC percentages (1%–20%) compared with EM-Seq v2 and at high-GC percentages (70%–100%) compared with bisulfite sequencing.