##### Library prep

[Illumina 5-Base DNA Prep](https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/illumina-5-base-dna-prep.html)

A single assay for comprehensive discovery of the 5-base genome (A, T, G, C, and 5mC), providing dual insights into the whole genome and methylome.

##### Sequencing

[NovaSeq 6000 System](https://www.illumina.com/systems/sequencing-platforms/novaseq.html)

Perform powerful high-throughput sequencing studies. Match data output and time to results to project needs.

[NovaSeq X Series](https://www.illumina.com/systems/sequencing-platforms/novaseq-x-plus.html)

Advanced chemistry, optics, and informatics combine to deliver exceptional sequencing speed and data quality, outstanding throughput, and scalability.

##### Data analysis

[DRAGEN secondary analysis](https://www.illumina.com/products/by-type/informatics-products/dragen-secondary-analysis.html)

Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing (NGS) data.

[Illumina Connected Multiomics](https://www.illumina.com/products/by-type/informatics-products/connected-multiomics.html)

A cloud-based multiomic analysis and interpretation software, enabling sample to insight workflow with visualizations, scalability, and secure data management.

Detect five DNA bases in a single assay while delivering concurrent genetic and epigenetic insights.

Sensitivity and precision plot for germline single nucleotide variant (SNV) calling shows Illumina 5-Base DNA Prep is very close to standard whole-genome sequencing (WGS) with Illumina DNA PCR-Free Prep, and higher than EM-Seq v2 and bisulfite sequencing.

Illumina 5-Base DNA Prep uses a one-step enzymatic process to convert only 5mC to T, yielding greater nucleotide diversity than traditional bisulfite treatment or enzymatic methylation sequencing (EM-Seq), in which unmethylated C are converted to T.

Illumina 5-Base DNA Prep offers highly accurate methylation measurement at CpG islands. Weighted methylation levels of individual CpG islands using Illumina 5-Base DNA Prep are highly concordant with EM-Seq v2 (left) and with bisulfite sequencing (right).

Illumina 5-Base DNA Prep shows ~20% more CpGs with at least 10× coverage compared to EM-Seq v2 and 68% more CpGs with at least 10× coverage compared to bisulfite sequencing for 450M clusters (900M paired-end reads).

Illumina 5-Base DNA Prep shows reduced GC bias with normalized coverage that is consistently higher at low-GC percentages (1%–20%) compared with EM-Seq v2 and at high-GC percentages (70%–100%) compared with bisulfite sequencing.